See https://hbctraining.github.io/Intro-to-ChIPseq/lessons/12_functional_analysis.html

BiocManager::install("ChIPseeker")
'getOption("repos")' replaces Bioconductor standard repositories, see '?repositories' for details

replacement repositories:
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a
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library(org.Hs.eg.db)
peakAnno <- annotatePeak(peakfile, tssRegion=c(-3000, 3000), TxDb=txdb)
>> loading peak file...              2021-12-23 20:14:39 
>> preparing features information...         2021-12-23 20:14:39 
>> identifying nearest features...       2021-12-23 20:14:40 
>> calculating distance from peak to TSS...  2021-12-23 20:14:41 
>> assigning genomic annotation...       2021-12-23 20:14:41 
>> assigning chromosome lengths          2021-12-23 20:15:08 
>> done...                   2021-12-23 20:15:08 

peakAnnoDf <- data.frame(peakAnno@anno)
peakEntrez <- peakAnnoDf$geneId
annotations_edb <- AnnotationDbi::select(EnsDb.Hsapiens.v75,
                                         keys = peakEntrez,
                                         columns = c("GENENAME"),
                                         keytype = "ENTREZID")
annotations_edb$ENTREZID <- as.character(annotations_edb$ENTREZID)
ego <- enrichGO(gene = peakEntrez, 
                    keyType = "ENTREZID", 
                    OrgDb = org.Hs.eg.db, 
                    ont = "BP", 
                    pAdjustMethod = "BH", 
                    qvalueCutoff = 0.05, 
                    readable = TRUE)

ekegg <- enrichKEGG(gene = peakEntrez,
                 organism = 'hsa',
                 pvalueCutoff = 0.05)
Reading KEGG annotation online:

Reading KEGG annotation online:
ekegg <- enrichKEGG(gene = peakEntrez,
                 organism = 'hsa',
                 pvalueCutoff = 0.05)
dotplot(ekegg)

promoter <- getPromoters(TxDb=txdb, upstream=3000, downstream=3000)
promoter <- getPromoters(TxDb=txdb, upstream=3000, downstream=3000)
tagMatrix <- getTagMatrix(peakAnno@anno, windows=promoter)
>> preparing start_site regions by ... 2021-12-22 21:54:07
>> preparing tag matrix...  2021-12-22 21:54:07 
plotAvgProf(tagMatrix, xlim=c(-3000, 3000), conf = 0.95, resample = 1000)
>> plotting figure...            2021-12-22 21:55:08 
>> Running bootstrapping for tag matrix...       2021-12-22 21:55:27 

plotPeakProf2(peak = peakAnno@anno, upstream = rel(0.2), downstream = rel(0.2),
              conf = 0.95, by = "gene", type = "body", nbin = 800,
              TxDb = txdb, weightCol = "V5",ignore_strand = F)
>> binning method is used...2021-12-22 21:55:45
>> preparing body regions by gene... 2021-12-22 21:55:45
>> preparing tag matrix by binning...  2021-12-22 21:55:45 
>> preparing matrix with extension from (TSS-20%)~(TTS+20%)... 2021-12-22 21:55:45
>> 52 peaks(1.028074%), having lengths smaller than 800bp, are filtered... 2021-12-22 21:55:57
>> Running bootstrapping for tag matrix...       2021-12-22 21:59:02 

intron_matrix <- getTagMatrix(peak = peakAnno@anno, 
                           TxDb = txdb,
                           upstream = rel(0.2),
                           downstream = rel(0.2), 
                           by = "intron",
                           type = "body",
                           nbin = 100,
                           )
>> binning method is used...2021-12-22 22:08:56
>> preparing body regions by intron... 2021-12-22 22:08:56
>> preparing tag matrix by binning...  2021-12-22 22:08:56 
>> preparing matrix with extension from (TSS-20%)~(TTS+20%)... 2021-12-22 22:08:56
>> 51 peaks(0.1213997%), having lengths smaller than 100bp, are filtered... 2021-12-22 22:09:11
intron_matrix <- getTagMatrix(peak = peakAnno@anno, 
                           TxDb = txdb,
                           upstream = rel(0.2),
                           downstream = rel(0.2), 
                           by = "intron",
                           type = "body",
                           nbin = 100,
                           )
>> binning method is used...2021-12-22 22:14:45
>> preparing body regions by intron... 2021-12-22 22:14:45
>> preparing tag matrix by binning...  2021-12-22 22:14:45 
>> preparing matrix with extension from (TSS-20%)~(TTS+20%)... 2021-12-22 22:14:46
>> 51 peaks(0.1213997%), having lengths smaller than 100bp, are filtered... 2021-12-22 22:15:03
plotPeakProf(tagMatrix = intron_matrix, conf = 0.95)
>> Running bootstrapping for tag matrix...       2021-12-22 22:20:50 

intron_matrix <- getTagMatrix(peak = peakAnno@anno, 
                           TxDb = txdb,
                           upstream = 1000,
                           downstream = 1000, 
                           by = "intron",
                           type = "start_site",
                           nbin = 250,
                           )
>> binning method is used...2021-12-22 22:32:52
>> preparing start_site regions by intron... 2021-12-22 22:32:52
>> preparing tag matrix by binning...  2021-12-22 22:32:52 
intron_matrix <- getTagMatrix(peak = peakAnno@anno, 
                           TxDb = txdb,
                           upstream = 1000,
                           downstream = 1000, 
                           by = "intron",
                           type = "start_site",
                           nbin = 250,
                           )
>> binning method is used...2021-12-22 22:32:57
>> preparing start_site regions by intron... 2021-12-22 22:32:57
>> preparing tag matrix by binning...  2021-12-22 22:32:57 
plotPeakProf(tagMatrix = intron_matrix, conf = 0.95)
>> Running bootstrapping for tag matrix...       2021-12-22 22:33:04 

plotPeakProf(tagMatrix = intron_matrix, conf = 0.95)
>> Running bootstrapping for tag matrix...       2021-12-22 22:47:02 

plotPeakProf(tagMatrix = exon_matrix, conf = 0.95)
>> Running bootstrapping for tag matrix...       2021-12-22 22:44:29 

exon_matrix <- getTagMatrix(peak = peakAnno@anno, 
                           TxDb = txdb,
                           upstream = 1000,
                           downstream = 1000, 
                           by = "exon",
                           type = "start_site",
                           nbin=250
                           )
>> binning method is used...2021-12-22 23:31:27
>> preparing start_site regions by exon... 2021-12-22 23:31:27
>> preparing tag matrix by binning...  2021-12-22 23:31:27 
exon_matrix <- getTagMatrix(peak = peakAnno@anno, 
                           TxDb = txdb,
                           upstream = 1000,
                           downstream = 1000, 
                           by = "exon",
                           type = "start_site",
                           nbin=250
                           )
>> binning method is used...2021-12-22 23:31:34
>> preparing start_site regions by exon... 2021-12-22 23:31:34
>> preparing tag matrix by binning...  2021-12-22 23:31:34 
plotPeakProf(exon_matrix, xlab="Exon start (5'->3')", ylab = "Peak Frequency", conf = 0.95, resample = 1000)
>> Running bootstrapping for tag matrix...       2021-12-22 23:31:48 

exon_matrix <- getTagMatrix(peak = peakAnno@anno, 
                           TxDb = txdb,
                           upstream = 1000,
                           downstream = 1000, 
                           by = "exon",
                           type = "end_site",
                           nbin=250
                           )
>> binning method is used...2021-12-22 23:31:49
>> preparing end_site regions by exon... 2021-12-22 23:31:49
>> preparing tag matrix by binning...  2021-12-22 23:31:49 
plotPeakProf(exon_matrix, xlab="Exon end (5'->3')", ylab = "Peak Frequency", conf = 0.95, resample = 1000)
>> Running bootstrapping for tag matrix...       2021-12-22 23:32:08 

utr_matrix <- getTagMatrix(peak = peakAnno@anno, 
                           TxDb = txdb,
                           upstream = 1000,
                           downstream = 1000, 
                           by = "3UTR",
                           type = "start_site",
                           nbin=250
                           )
>> binning method is used...2021-12-22 23:34:17
>> preparing start_site regions by 3UTR... 2021-12-22 23:34:17
>> preparing tag matrix by binning...  2021-12-22 23:34:17 
utr_matrix <- getTagMatrix(peak = peakAnno@anno, 
                           TxDb = txdb,
                           upstream = 1000,
                           downstream = 1000, 
                           by = "3UTR",
                           type = "start_site",
                           nbin=250
                           )
>> binning method is used...2021-12-22 23:34:27
>> preparing start_site regions by 3UTR... 2021-12-22 23:34:27
>> preparing tag matrix by binning...  2021-12-22 23:34:27 
plotPeakProf(utr_matrix, xlab="3' UTR start (5'->3')", ylab = "Peak Frequency", conf = 0.95, resample = 1000)
>> Running bootstrapping for tag matrix...       2021-12-22 23:34:33 

utr_matrix <- getTagMatrix(peak = peakAnno@anno, 
                           TxDb = txdb,
                           upstream = 1000,
                           downstream = 1000, 
                           by = "3UTR",
                           type = "end_site",
                           nbin=250
                           )

plotPeakProf(utr_matrix, xlab="3' UTR end (5'->3')", ylab = "Peak Frequency", conf = 0.95, resample = 1000)
utr_matrix <- getTagMatrix(peak = peakAnno@anno, 
                           TxDb = txdb,
                           upstream = 1000,
                           downstream = 1000, 
                           by = "5UTR",
                           type = "start_site",
                           nbin=250
                           )
>> binning method is used...2021-12-22 23:36:22
>> preparing start_site regions by 5UTR... 2021-12-22 23:36:22
>> preparing tag matrix by binning...  2021-12-22 23:36:22 
utr_matrix <- getTagMatrix(peak = peakAnno@anno, 
                           TxDb = txdb,
                           upstream = 1000,
                           downstream = 1000, 
                           by = "5UTR",
                           type = "start_site",
                           nbin=250
                           )
>> binning method is used...2021-12-22 23:36:29
>> preparing start_site regions by 5UTR... 2021-12-22 23:36:29
>> preparing tag matrix by binning...  2021-12-22 23:36:29 
plotPeakProf(utr_matrix, xlab="5' UTR start (5'->3')", ylab = "Peak Frequency", conf = 0.95, resample = 1000)
>> Running bootstrapping for tag matrix...       2021-12-22 23:36:35 

utr_matrix <- getTagMatrix(peak = peakAnno@anno, 
                           TxDb = txdb,
                           upstream = 1000,
                           downstream = 1000, 
                           by = "5UTR",
                           type = "end_site",
                           nbin=250
                           )

plotPeakProf(utr_matrix, xlab="5' UTR end (5'->3')", ylab = "Peak Frequency", conf = 0.95, resample = 1000)
plotPeakProf(gene_matrix, xlab="Gene start (5'->3')", ylab = "Peak Frequency", conf = 0.95, resample = 1000)
>> Running bootstrapping for tag matrix...       2021-12-23 20:16:29 

plotPeakProf(gene_matrix, xlab="Gene end (5'->3')", ylab = "Peak Frequency", conf = 0.95, resample = 1000)
>> Running bootstrapping for tag matrix...       2021-12-23 20:16:06 

---
title: "R Notebook"
output: html_notebook
---

See https://hbctraining.github.io/Intro-to-ChIPseq/lessons/12_functional_analysis.html

```{r}
BiocManager::install("ChIPseeker")
BiocManager::install("GO.db")
BiocManager::install("DO.db")
BiocManager::install("EnsDb.Hsapiens.v75")
BiocManager::install("clusterProfiler")
BiocManager::install("org.Hs.eg.db")
BiocManager::install("TxDb.Hsapiens.UCSC.hg38.knownGene")
```


```{r}
library(ChIPseeker)
library(TxDb.Hsapiens.UCSC.hg38.knownGene)
library(EnsDb.Hsapiens.v75)
library(clusterProfiler)
library(AnnotationDbi)
library(org.Hs.eg.db)
```

```{r}
txdb <- TxDb.Hsapiens.UCSC.hg38.knownGene
peakfile <- "../data/raw/eclip/RPL22-ZR751_clearCLIP.pool.tag.uniq.del.CIMS.fdr10.f10.bed"
peakAnno <- annotatePeak(peakfile, tssRegion=c(-3000, 3000), TxDb=txdb)
```
```{r}
plotAnnoBar(peakAnno)
```

```{r}
plotDistToTSS(peakAnno)
```
```{r}
peakAnnoDf <- data.frame(peakAnno@anno)
peakEntrez <- peakAnnoDf$geneId
annotations_edb <- AnnotationDbi::select(EnsDb.Hsapiens.v75,
                                         keys = peakEntrez,
                                         columns = c("GENENAME"),
                                         keytype = "ENTREZID")
annotations_edb$ENTREZID <- as.character(annotations_edb$ENTREZID)
```

```{r}
ego <- enrichGO(gene = peakEntrez, 
                    keyType = "ENTREZID", 
                    OrgDb = org.Hs.eg.db, 
                    ont = "BP", 
                    pAdjustMethod = "BH", 
                    qvalueCutoff = 0.05, 
                    readable = TRUE)
```

```{r}
dotplot(ego, showCategory=25)

```

```{r}
ekegg <- enrichKEGG(gene = peakEntrez,
                 organism = 'hsa',
                 pvalueCutoff = 0.05)

dotplot(ekegg)
```

```{r}
promoter <- getPromoters(TxDb=txdb, upstream=3000, downstream=3000)
exon <- getI(TxDb=txdb, upstream=3000, downstream=3000)
tagMatrix <- getTagMatrix(peakAnno@anno, windows=promoter)

```

```{r}
plotAvgProf(tagMatrix, xlim=c(-3000, 3000), conf = 0.95, resample = 1000)
```

```{r}
plotPeakProf2(peak = peakAnno@anno, upstream = rel(0.2), downstream = rel(0.2),
              conf = 0.95, by = "gene", type = "body", nbin = 800,
              TxDb = txdb, weightCol = "V5",ignore_strand = F)
```

```{r}
intron_matrix <- getTagMatrix(peak = peakAnno@anno, 
                           TxDb = txdb,
                           upstream = rel(0.2),
                           downstream = rel(0.2), 
                           by = "intron",
                           type = "body",
                           nbin = 100,
                           )

plotPeakProf(tagMatrix = intron_matrix, conf = 0.95)
```

```{r}
intron_matrix <- getTagMatrix(peak = peakAnno@anno, 
                           TxDb = txdb,
                           upstream = 1000,
                           downstream = 1000, 
                           by = "intron",
                           type = "start_site",
                           nbin = 250,
                           )

plotPeakProf(tagMatrix = intron_matrix, conf = 0.95)
```
```{r}
intron_matrix <- getTagMatrix(peak = peakAnno@anno, 
                           TxDb = txdb,
                           upstream = 1000,
                           downstream = 1000, 
                           by = "intron",
                           type = "end_site",
                           nbin = 250,
                           )

plotPeakProf(tagMatrix = intron_matrix, conf = 0.95)
```

```{r}
exon_matrix <- getTagMatrix(peak = peakAnno@anno, 
                           TxDb = txdb,
                           upstream = 1000,
                           downstream = 1000, 
                           by = "exon",
                           type = "start_site",
                           nbin = 250,
                           )

plotPeakProf(tagMatrix = exon_matrix, conf = 0.95)
```
```{r}
exon_matrix <- getTagMatrix(peak = peakAnno@anno, 
                           TxDb = txdb,
                           upstream = 1000,
                           downstream = 1000, 
                           by = "exon",
                           type = "start_site",
                           nbin=250
                           )

plotPeakProf(exon_matrix, xlab="Exon start (5'->3')", ylab = "Peak Frequency", conf = 0.95, resample = 1000)
```
```{r}
exon_matrix <- getTagMatrix(peak = peakAnno@anno, 
                           TxDb = txdb,
                           upstream = 1000,
                           downstream = 1000, 
                           by = "exon",
                           type = "end_site",
                           nbin=250
                           )

plotPeakProf(exon_matrix, xlab="Exon end (5'->3')", ylab = "Peak Frequency", conf = 0.95, resample = 1000)
```

```{r}
utr_matrix <- getTagMatrix(peak = peakAnno@anno, 
                           TxDb = txdb,
                           upstream = 1000,
                           downstream = 1000, 
                           by = "3UTR",
                           type = "start_site",
                           nbin=250
                           )

plotPeakProf(utr_matrix, xlab="3' UTR start (5'->3')", ylab = "Peak Frequency", conf = 0.95, resample = 1000)
```

```{r}
utr_matrix <- getTagMatrix(peak = peakAnno@anno, 
                           TxDb = txdb,
                           upstream = 1000,
                           downstream = 1000, 
                           by = "3UTR",
                           type = "end_site",
                           nbin=250
                           )

plotPeakProf(utr_matrix, xlab="3' UTR end (5'->3')", ylab = "Peak Frequency", conf = 0.95, resample = 1000)
```

```{r}
utr_matrix <- getTagMatrix(peak = peakAnno@anno, 
                           TxDb = txdb,
                           upstream = 1000,
                           downstream = 1000, 
                           by = "5UTR",
                           type = "start_site",
                           nbin=250
                           )

plotPeakProf(utr_matrix, xlab="5' UTR start (5'->3')", ylab = "Peak Frequency", conf = 0.95, resample = 1000)
```

```{r}
utr_matrix <- getTagMatrix(peak = peakAnno@anno, 
                           TxDb = txdb,
                           upstream = 1000,
                           downstream = 1000, 
                           by = "5UTR",
                           type = "end_site",
                           nbin=250
                           )

plotPeakProf(utr_matrix, xlab="5' UTR end (5'->3')", ylab = "Peak Frequency", conf = 0.95, resample = 1000)
```

```{r}
gene_matrix <- getTagMatrix(peak = peakAnno@anno, 
                           TxDb = txdb,
                           upstream = 1000,
                           downstream = 1000, 
                           by = "gene",
                           type = "start_site",
                           nbin=250
                           )

plotPeakProf(gene_matrix, xlab="Gene start (5'->3')", ylab = "Peak Frequency", conf = 0.95, resample = 1000)
```

```{r}
gene_matrix <- getTagMatrix(peak = peakAnno@anno, 
                           TxDb = txdb,
                           upstream = 1000,
                           downstream = 1000, 
                           by = "gene",
                           type = "end_site",
                           nbin=250
                           )

plotPeakProf(gene_matrix, xlab="Gene end (5'->3')", ylab = "Peak Frequency", conf = 0.95, resample = 1000)
```